Composite

Part:BBa_K4409014:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


gRNA (CT+ BBa_K3859000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We utilized the BBa_K4409000 sequence to construct the Cas12 handle and BBa_K3859000 designed by GreatBay_SZ to construct the guide sequence. Since there is no trigger sequence serving as a switch for whole sequence, as long as the dsDNA for the guide is present, the igRNA will always be turned on. This sequence can serve as a positive control group to compare the results of different guide sequence being utilized.


Source

The sequence we designed is based on BBa_K4409000 for Cas12 handle with CT present, and BBa_K3859000 for the guide sequence.

References