![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4409014:Design
Designed by: Kexin Fei Group: iGEM22_Worldshaper-HZ (2022-09-20)
gRNA (CT+ BBa_K3859000)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We utilized the BBa_K4409000 sequence to construct the Cas12 handle and BBa_K3859000 designed by GreatBay_SZ to construct the guide sequence. Since there is no trigger sequence serving as a switch for whole sequence, as long as the dsDNA for the guide is present, the igRNA will always be turned on. This sequence can serve as a positive control group to compare the results of different guide sequence being utilized.
Source
The sequence we designed is based on BBa_K4409000 for Cas12 handle with CT present, and BBa_K3859000 for the guide sequence.